Vol. 4 No. 2 (2026), Articles
Vol. 4 No. 2 (2026)

PSEUDOMONAS AERUGINOSA BIOPHIL FORMATION MECHANISMS AND ANTIBIOTIC RESISTANCE RELATING TO CURRENT ISSUES

Articles
Published 2026-02-13
Buriyev Muhammadali Gayrat oglu
Termez University of Economics and Service Faculty of Medicine Department of Medical Preventive Sciences Lecturer in Microbiology
Akhmedov Ulugbek Xolbek oglu
Termez University of Economics and Service, Department of Fundamental Medical Sciences, Assistant
Khushboqov Shohruh Ulug’bek oglu
Termez University of Economics and Service Student of the Faculty of Medicine
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Keywords

Pseudomonas aeruginosa, biofilm, quorum sensing, antibiotic resistance, EPS matrix, MIC, furanone-AHL, efflux pump, persister cells, Crystal Violet assay.

How to Cite

PSEUDOMONAS AERUGINOSA BIOPHIL FORMATION MECHANISMS AND ANTIBIOTIC RESISTANCE RELATING TO CURRENT ISSUES. (2026). MEDICINE, PEDAGOGY AND TECHNOLOGY: THEORY AND PRACTICE, 4(2), 46-56. https://universalpublishings.com/~niverta1/index.php/mpttp/article/view/16685
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Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that forms biofilms and is highly resistant to antibiotics. This study investigated the steps of bifilm formation, the mechanism of quorum sensing, and the antibiogram profile using a novel approach. Crystal violet assay and MIC detection methods were used to determine the 72-hour biofilm density (OD 0.912) and 10–128-fold resistance to antibiotics. Furanone-AHL quorum sensing inhibitor was shown to have an 80% biofilm reduction effect. The results provide a basis for a biofilm-fighting strategy as a promising solution for QS-opponent therapy.

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References

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FOYDALANILGAN SAYTLAR

1. NCBI PubMed — Biofilm va Pseudomonas aeruginosa qidirish: https://pubmed.ncbi.nlm.nih.gov/?term=pseudomonas+biofilm

2. WHO — Antimicrobial Resistance: https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance

3. CLSI — Clinical Laboratory Standards Institute: https://www.clsi.org/

4. CDC — Antibiotic Resistance Threats: https://www.cdc.gov/antimicrobialresistance/