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Abstract
Hydatid disease is an important emerging neglected disease worldwide, with significant geographic variation in seroprevalance. The disease is commonly diagnosed on basis of clinical suspicion, imaging and serology. This study was conducted to evaluate our experience with serology in diagnosing hydatid disease in terms of identifying the numbers of patients testing positive for antibodies against Echinococcus, and to study their clinical profile. This study included all patients who tested for anti-Echinococcus IgG antibodies by ELISA in the years 2011-2013. The clinical characteristics of seropositive patients were contrasted with seronegative patients, and the test results were correlated with radiological findings and, where performed, with histopathological studies. Analysis was performed on SPSS. Out of 31 patients evaluated by serology for Echinococcosis during the study period, 7 (22.58%) were seropositive. Liver was the most frequent site involved (24 patients; 77.77%). Second organ the lung 5(16.13%) bone 1(3.23%) and the optic 1(3.22%).Of the 29 patients who also underwent biopsy, all the seven patients with positive biopsy were seropositive, suggesting 100% sensitivity. The specificity was 83.33%, but this might be a lower estimate due to potentially high rate of false-negative biopsies, as all the seropositive patients also had imaging features suggestive of disease. The positive and negative predictive values were 77.77% and 90.90%, respectively. ELISA for detection of antiEchinococcus antibodies is a simple serological test that helps in correlation with imaging finding in the diagnosis and subsequent management of hydatid disease.
References
Ameur A, Lezrek M, Boumdin H, et al.( 2002;) Hydatid cyst of the kidney based on a series of 34 cases. Prog Urol 12 (3):409- 14.
Ataoglu H, Uckan S, Oz G, Altinörs N. (2002). Maxillofacial hydatid cyst.J Oral Maxillofac Surg.; 60: 454-456
Barbieri, M., V. Fernandez, G, Gonzalez, V. M., and Nieto A. (1998). Diagnostic evaluation of a synthetic peptide derived from a novel antigen B subunit as related to other available peptides and native antigens used for serology of cystic hydatidosis. Parasite Immunology Journal 20:51-61
Bouckaert MM, Raubenheimer EJ, Jacobs FJ.(2000). Maxillofacial hydatid cysts.Oral Surg Oral Med Oral Pathol Oral RadiolEndod ; 89: 338-342.
Brunetti E, Kern P, Vuitton DA, (2010). ―Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans,‖ Acta Tropica, vol. 114,no. 1, 1– 16,
Budke CM, Deplazes P, Torgerson PR (2006) Global socioeconomic impact of cystic echinococcosis. Emerg Infect Dis 12:296–303
Budke CM, Carabin H, Ndimubanzi PC, Nguyen H, Rainwater E, Dickey M, Bhattarai R, Zeziulin O, Qian M-B (2013) A systematic review of the literature on cystic echinococcosis frequency worldwide and its associated clinical manifestations. Am J Trop Med Hyg 88:1011– 1027.
Cooney, RM, Flanagan KP, & Zehyle, E. (2004) Review of surgical management of cystic hydatid disease in a resource limited setting: Turkana, Kenya. Euro J of
Gastroenterology & Hepatology. 16(11): 1233– 1236
Despommier, D; RW Gwadz, PJ Hotez and CA Knirsch. (2006). Parasitic Diseases. 5th ed. New York: Apple Trees Production, LLC. 2006
Djuricic SM, Grebeldinger S, Kafka DI, Djan I, Vukadin M, Vasiljevic ZM (2010) Cystic echinococcosis in children—the seventeen-year experience of two large medical centers in Serbia. Parasitol Int 59:257–261
Dopchiz MC, Elissondo MC, Rossin MA, Denegri G (2007) Hydatidosis cases in one of Mar del Plata hospitals, Buenos Aires, Argentina. Rev Soc Bras Med Trop 40:635–639
Echinococcosis, WHO Headquarter Geneva, Switzerland 15 - 16 December 2016, 28 March 2017
Eckert, J. and Deplazes, P. (2004). Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern,‖ Clinical Microbiology Reviews, 17(1):107– 135.
Gharde P, Wagh DD, Patil A. (2012). Left renal hydatid cyst presenting as hematuria and macroscopic hydatiduria since last ten years. Trop Parasitol; 2:58-60.
Florea,A., Vlad,L., Cozma,V., Coroiu,Z. (2009). Serological diagnosis of cystic echinococcosis by the ELISA technique, in the cases hospitalized in the surgical clinic no. III and internal medicine no. III of Cluj-Napoca, during October 2006 –December 2009. Sci. Parasitol., 12(3):167-71.
Gogus, C, Safak, M, Baltaci, S, Turkjolmez, Z. ((2003). Isolated renal hydatidosis: Experience with 20 cases. J. Urol. 169: 186- 189
Gottstein B, (1992). Molecular and immunological diagnosis of Echinococcosis . clin Microbiol., Rev. 5: 248-261
Grosso G, Gruttadauria S, Biondi A, Marventano S, Mistretta A. (2012). Worldwide epidemiology of liver hydatidosis including the Mediterranean area. World J Gastroenterol; 18:1425-37.
Hafsa C, Ben Alaya T, Kriaa S, Jerbi S, Golli M, Hamdi MH, et al.( 2008). Maxillo-facial location of hydatic cyst.Ann OtolaryngolChirCervicofac.; 125: 160- 163.
Hashemi, S. Tahar, G.R. and Razmi, G.R. (2009) antibody response against hydatid fluid, Protoscoleces and all body of Echinococcus granulosus antigens in lambs. Iranian Journal of Veterinary Researches shiaz University. Volume 10, no. 3. Serial No. 28, 2009.
Herrera, A.; Martinez A.A. (2003) Extraspinal bone hydatidosis, J Bone Joint Surg Am., 85 1790- 1794.
Hosch W, Junghanss T, Stojkovic M, Brunetti E, Heye T, et al. (2008) Metabolic viability assessment of cystic echinococcosis using high-field 1H MRS of cyst contents. NMR
Larrieu EJ, Frider B (2001) Human cystic echinococcosis: contributions to the natural history of the disease. Am J Trop Med Parasitol 95:679–687
Lightowlers, M. W.; Gauci, C. G. ;Chow, C. ;Drew, D. R. ;Gane, C. S. M.; Heath, D. D.; Jackson, D. C.; Dadley, D. L. and Read, A. J. (2003). Molecular and genetic characterization of the host-protective oncosphere antigen of taeniid cestode parasites. Inter. J. for Paras.(331):1207-1217.
Lahmar S, Debbek H, Zhang LH, et al. (May 2004). "Transmission dynamics of the Echinococcus granulosus sheep-dogstrain (G1 genotype) in camels in Tunisia". Vet. Parasitol. 121 (1-2): 151-6.
Li D, Gao Q, Liu J, Feng Y, Ning W, Dong Y, etal.( 2015). Knowledge, attitude, and practices (KAP) and risk factorsanalysis related to cystic echinococcosis among residentsin Tibetan communities, Xiahe County, Gansu Province,China. Acta Trop; 147:17-22
Lightowlers MW, Gottstein B.(1995). Echinococcosis/hydatidosis: antigens, immunological and molecular diagnosis. In: Thompson RCA, Lymbery AJ, editors. Echinococcus and hydatid disease. Wallingford, UK: CAB International; 355-410.
Luke N, Francis E, MichealLO, Clovice K, Ludwig S,Micheal O, (2013). A survey of potential risk factors associatedwith cystic echinococcosis in pastoral communities in Kasese distract, Uganda. Adv Trop Med Public Health Int 2013;3:10-24.
Kurul IC, Topcu S, Altinok T, Yazucu U, Tastepe I, Kaya S, Cetin G (2002) One-stage operation for hydatid disease of lung and liver: principles of treatment. J Thorac Cardiovasc Surg 124:1212– 1215
Jenkins, D. J. (2005). Hydatid control in Australia: where it began, what we have achieved and where to from here. International Journal forParasitology, 35(7):733–740
Kaddah MH,Maher KM, Hassanein HI, Farrag AL, Shaker ZA, Khalafallah AM, (1992).evaluation of different immunodiagnosis techniques for diagnosis of hydatidosis in Egypt. J Egypt Soc. Paraitol. 22: 653-665.
Katilmiş H, Oztürkcan S, Ozdemir I, AdadanGüvenç I, Ozturan S,(2007). Primary hydatid cyst of the neck. Am J Otolaryngol.; 28: 205-207.
Khanfar N., (2004). Hydatid disease: a review and update. Current Anaesthesia & Critical Care, 15, 173– 183.
King S and Hutchinson GW (2007). Hydatids – you, too, can be affected. Primefact 475 NSW Department of Primary Industries.
Manzano-Román,R., Sánchez-Ovejero,C., Hernández- González ,A., Casulli,A., Siles-Lucas,M. 2015. Serological diagnosis and follow up of human cystic echinococcosis: a new hope for the future? Biomed.Res.Int., 428205.
McManus DP, Zhang W, Li J, Bartley PB (October 2003). "Echinococcosis". Lancet. 362 (9392): 1295– 304.
Meziane, A. Bachechar N., Benkirane, A. Ouadfel, J. (1987). Hydatid cyst of pelvis: a case report Acta Orthop Belg., 53 , 517-519
Moro P., Schantz PM. (2009). - Echinococcosis: a review- International Journal of Infectious Diseases, 13, 125— 133.
Nyakarahuka L, Lalobo-Oryema M, Kankya C, SiefertL,Ocaido M, Ejobi F, (2012). Knowledge, attitude and practices towards cystic echinococcosis in pastoral communi-ties in Kasese district. Adv Trop Med Public Health Int ;2:32-9
Oba P, Ejobi F, Omadang L, Chamai M, Okwi AL, OthienoE, etalm (2016).Prevalence and risk factors of Echinococcus granulosus infection in dogs in Moroto and Bukedea districts in Uganda. Trop Anim Health Prod; 48:249-54.
Ozkan, H. Y. Dogramaci, O. Kose, E. Esen, H. Erdem, M. Komurcu; (2008). Primary hydatid cyst of the humerus Ann Acad Med., 37, pp. 440-441
Permin and Hanser, J. W. (2004). Review of echinococcosis a zoonotic parasitic disease. http: /www.fao.org/docrep/t 1300t/t/1300Tom.htm.
Pierangeli NB, Soriano SV, Roccia I, Giménez J, Lazzarini LE, Grenóvero MS, Menestrina C, Basualdo JA (2007) Heterogeneous distribution of human cystic echinococcosis after a long-term control program in Neuquén, Patagonia Argentina. Parasitol Int 56:149– 155
Romig, T, Omer, RA, Zeyhle, E et al.(2011)‘ Echinococcosis in sub-Saharan Africa: emerging complexitiy. Vet Parasitol. 2011; 181: 43–47.
Raubenheimer EJ, Jacobs FJ. (2000); Maxillofacial hydatid cysts.Oral Surg Oral Med Oral Pathol Oral Radiology Endodontology. 89: 338-342.
Sadjjadi SM.(2006). Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int.; 55 Suppl: S197-S202.
Sarkari, B.,Rezaei,Z. (2015). Immunodiagnosis of human hydatid disease: where do we stand? World J.Methodol., 5(4):185-95.
Schantz PM, Wang H, Qiu J, Liu FJ, Saito E, Emshoff A, etal,(2003). Echinococcosis on the Tibetan Plateau: Prevalence and risk factors for cystic and alveolar echinococcosis in Tibetan populations in Qinghai Province, China.Parasitology; 127 Suppl: S109-20
Shah KJ, Ganpule AP, Desai MR. (2009).Isolated renal hydatid cyst managed by laparoscopic transperitoneal nephrectomy. Indian J Urol; 25:531-3.
Stojkovic ,M., Rosenberger,K., Kauczor,H.U.,
Junghanss,T., Hosch,W. (2012). Diagnosing and staging of cystic echinococcosis: how do CT and MRI perform in comparison to ultrasound? PloSNegl.Trop.Dis., 6(10):e1880.
Tenguria RK, NAIK MI. (2014). Evaluation of human cystic echinococcosis before and after surgery and chemotherapy by demonstration of antibodies in serum. Annals of parasitology, 60.4.
Thoma, D. and Weible, A. K. (2006). Echinococcus multilocularis a zoonosis of anthropogenic environments. Journal of Helminthology,80 (2): 207– 212.
Torgerson PR, Heath DD (2003). "Transmission dynamics and control options for Echinococcus granulosus". Parasitology. 127 (7): S143–58.
Turgut AT , Ödev K , Kabaalioğlu A , Bhatt S , Dogra VS,(2009). Multitechnique evaluation of renal hydatid disease. AJR Am J Roentgenol; 192(2):462–467
Tuzun, M. Hekimoglu. (2002), CT findings in skeletal echinococcosis Acta Radiol., 43 p. 533
Vatankhah, A., Assmar, M., Shokrgozar, M.A., Taghavi Hussein, S.A. and Esmaeili Rastaghi, A.R (2004). Introduction of an Indirect Haemagglutination Test as a rapid Diagnostic method in Comparison with ELISA using antigen B for diagnosis of Human Hydatid Disease. Iranian Journal public Health, volume 33, No. 4,pp.16-25
Wachira TM, Macpherson CNL, Gathuma JM, (1991). Release and survival of Echinococcus eggs in different environments in Turkana and their possible impact on the incidence of hydatidosis in man and livestock. J. Hemintholol.; 65:55-61
Wattal, C,Malla N, Khan IA, Agarwal SC. (1986). Comparative evaluation of enzyme linked immunosorbent assay for the diagnosis of pulmonary Echinococcosis. J Clin. Microbiol. 24: 41-46
Wang Q, Huang Y, Huang L, Yu W, He W, Zhong B, etal, (2014). Review of risk factors for human echinococcosis prevalence on the Qinghai-Tibet Plateau, China: A prospective for control options. Infect Dis Poverty 2014;3:3
Wani NA, Kousar TL, Gojwari T, Robbani I, Singh M, zan AR, et al.( 2011). Computed tomography findings in cerebral hydatid disease. Turkish Neurosurg; 21: 347-351.
Wuestenberg,J., Gruener,B., Oeztuerk,S., Mason,R.A., Haenle,M.M., Graeter,T.et al. (2014). Diagnostics in cystic echinococcosis: serology versus ultrasonography. Turk.J.Gastroenterol., 25(4):398-404.
Yang YR, Ellis M, Sun T, Li Z, Liu X, Vuitton DA, etal ,(2006).Unique family clustering of human echinococcosis cases ina Chinese community. Am J Trop Med Hyg; 74:487-94.
Zlitini, M. K. Ezzaoula, H. Lebib, M. Karray, M. Kooli, M. Mesteri,(2001). Hydatid cyst of bone:diagnosis and treatment World J Surg., 25 ; 75-82
Wuestenberg,J., Gruener,B., Oeztuerk,S., Mason,R.A., Haenle,M.M., Graeter,T.et al. (2014). Diagnostics in cystic echinococcosis: serology versus ultrasonography. Turk.J.Gastroenterol., 25(4):398-404.
Zhang,W., Li,J., McManus,D.P. (2003). Concepts in immunology and diagnosis of hydatid disease. Clin. Microbiol.Rev., 16(1):18-36.
Zhang,W., Wen,H., Li,J., Lin,R., McManus,DP. (2012). Immunology and immunodiagnosis of cystic echinococcosis: an update. Clin.Dev.Immunol., 101895. Available from: doi:10.1155/2012/101895
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